The xfp extracellular flux analyzer is built on innovative and proven xf technology for live cellbased assays of energy metabolism. Bioenergetic analysis of intact mammalian cells using the. Xfe extracellular flux analyzers simultaneously interrogate the two major energy producing pathways of the cell mitochondrial respiration and glycolysis in a microplate, in realtime. The following items are included in the xfe24 instrument and controller boxes instrumentcontroller quantity. Seahorse xf cell mito stress test kit user guide agilent. Flux release another plugin pure analyser now 64bit, aax. The optimal number of each cell type to be plated per well must be determined empirically. View margaret lius profile on linkedin, the worlds largest professional community. Please note that the following four issues are critical in order to obtain reproducible and interpretable data. Extracellular flux an overview sciencedirect topics. The seahorse xf prep station is a compact, benchtop, fluid management system that prepares your culture plates for assay with the seahorse xfe or xf extracellular flux analyzers, saving valuable lab time, improving cvs, and increasing the sensitivity of your ecar measurements. Seahorse xf96 cell culture microplates seahorse xf calibrant 500 ml also required, but not included. The seahorse bioscience xf96 extracellular flux analyzer is an instrument that simultaneously measures two energy yielding pathways.
The seahorse extracellular flux xf analyzer seahorse bioscience is a powerful tool for measuring glycolysis and oxidative phosphorylation through oxygen consumption simultaneously in the same cells. France, les ulis, france as per the manufacturers instructions and as described in 27. Ocr in primary hepatocytes was measured 3 d after sirna application using seahorse bioscience xf96 extracellular flux analyzer. Xfe extracellular flux analyzer user guide version 2. The xf96 extracellular flux analyzer seahorse bioscience, north billerica, ma, usa was utilized to measure the extracellular flux changes of oxygen and protons in the media surrounding the adherent cells cultured onto the polydlysine coated xf96 well microplates. Seahorse xfp analyzer user manual 7 overview the agilent xfp analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a miniplate.
The xf96 extracellular flux analyzer seahorse bioscience, north billerica, ma, usa was utilized to measure the extracellular flux changes of oxygen and protons in the media surrounding the adherent cells cultured onto the polydlysine coated xf96well microplates. Compact and easy to use, the xfp analyzer is designed to perform standard xf assays, and is ideal for pairwise comparisons. Net arctic cat xf 7000 operators manual download arctic cat xf 7000 operators. Seahorse xf24 extracellular flux analyzer the seahorse xf24 is a revolutionary instrument for studies of cellular energy metabolism. We have 1 seahorse xf96 manual available for free pdf download. Differential effects of fxr or tgr5 activation in cholangiocarcinoma progression. Bk precision 4052 function generator the 4052 is a function generator from bk precision. The amount of time to record the flux of analytes in the transient. Agilent seahorse xf cell mito stress test kit user guide. This instrument and similar technological innovations have transformed the study of cellular.
S1 tracer portable xrf analyzer user manual june 2008 415 north quay kennewick, wa 99336 5097839850 advanced test equipment rentals. We have 1 seahorse xf96 manual available for free pdf. Advances in the quantification of mitochondrial function in primary human immune cells through extracellular flux analysis. Extracellular flux analyzer and prep station brand. Together with a seahorse xfe24, xf96 or xfe96 analyzer, the seahorse. This kind of signal generator uses integrated circuits to create waveforms.
Margaret liu software engineer lockerdome linkedin. View online or download seahorse xf96 installation and operation manual. Mitochondrial oxygen metabolism in primary human lens. My only regret with the pure analyzer system is that the information provided in the user manual is often meager. Seahorse xf96 installation and operation manual pdf download. Agilent seahorse xf realtime atp rate assay kit user guide. For use with seahorse xfxfe96 and xfe24 extracellular flux analyzers. The new generation of integra liquid handling tools uniquely bridge the gap between the power and productivity of automated liquid handlers and the simplicity and ease of use of manual systems. First, tumor volume was measured by mri one week after the orthotopic implantation of ccas. Xf96 or xfe96 analzyer or 500 l if using the xf24 or xfe24 analyzer. Seahorse xf e 24 extracellular flux analyzerbased analysis of cellular respiration in. The role of metabolism in cellular and physiological processes from cardiovascular function to immune response is well established, with many diseases now linked to metabolic dysfunction or reprogramming. Seahorse xfe 24 extracellular flux analyzerbased analysis of cellular respiration in caenorhabditis elegans.
The xf24 can also measure respiration of isolated mitochondria. Seahorse xfe analyzers from agilent cell analysis selectscience. Analysis of lymphocyte mitochondrial function has been facilitated by introduction of 96well format extracellular flux xf96 analyzers, but the technology remains imperfect for analysis of human lymphocytes. This user manual uses the symbols below to draw your specific attention to the remaining dangers associated with proper use, and to emphasize important technical requirements. Xfp extracellular flux analyzer user manual institute of anatomy. Xf67514new 2020 canon usa inc xf405download canon usa inc. Apr 26, 2017 metabolic studies were performed using the xf96 extracellular flux analyzer seahorse bioscience, north billerica, ma, usa which enables the simultaneous realtime measurement of aerobic glycolysis quantified by the extracellular acidification rate ecar and mitochondrial respiration quantified by the oxygen consumption rate ocr. Measurement of mitochondrial respiration and glycolysis allows energetic phenotyping of cells, in order to assess functional changes. The cellular oxygen consumption rate ocr data were obtained using an xf96 extracellular flux analyzer from seahorse bioscience. The volume was calculated from manual segmentation of the mri images using the imagej 1.
Circulating reentrant waves promote maturation of hipsc. This is what they say today we release the pure analyzer system sp3, introducing aax and 64bit compatibility. Apr 04, 2019 here is a best of collection of links to manuals, tools, and other resources for agilent cell analysis products. Lily chen frontend infrastructure engineer slack linkedin. Seahorse bioscience sells instruments and consumables. The xfp analyzer simultaneously measures the two major energy pathways of the cell mitochondrial respiration and glycolysis in live cells using label. The pure analyzer from flux is a big application with loads of options that displays and measures your audio in so many ways separate app. View and download seahorse xf96 installation and operation manual online.
Xfp extracellular flux analyzer user manual uzh institute of. The xfp analyzer utilizes patented transient microchambers which enable sensitive, precise, and nondestructive extracellular flux measurements in minutes. For the xf glycolysis stress test, assay medium with useradded glutamine is recommended. Bk precision 4052 function generator the 4052 is a function. Scanning seahorse bioscience xf96 cell culture microplates. Using xf technology, scientists can quickly and easily obtain functional metabolic data and gain a. Integra has announced that it will display its range of productive, handheld liquid handling tools at the inaugural european lab automation congress to be held in hamburg june 30th july 1st 2011. Preliminary experiments were carried out to prove that hescs and ipscs maintained the expression of key pluripotency. Seahorse xf96 installation and operation manual pdf. Metabolic studies were performed using the xf96 extracellular flux analyzer seahorse bioscience, north billerica, ma, usa which enables the simultaneous realtime measurement of aerobic glycolysis quantified by the extracellular acidification rate ecar and mitochondrial respiration quantified by the oxygen consumption rate ocr. Seahorse xf analyzers measure oxygen consumption rate ocr and extracellular acidification rate ecar of live cells in a multiwell plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis.
It covers the interface views, connectivity and suggestions on techniques for using the app in the studio and on stage. Agilent seahorse xf mito fuel flex test kit user manual. A function generator is a type of electronic test equipment that produces sine, triangular, square, and sawtooth shape waveforms. Seahorse xfe96 analyzers measure ocr and ecar of live cells in a 96well plate format. Extracellular flux assay was performed with a seahorse xf96 extracellular flux analyzer seahorse bioscience according to. Immunosuppressive capacity of mesenchymal stem cells.
Live quantification of cellular and mitochondrial bioenergetics was performed using seahorse xf96 extracellular flux analyzer seahorse bioscience, billerica, ma. The user must not use this equipment for any other purpose than that stated. Seahorse xfp extracellular flux analyzer user manual. Lisa2 cells were seeded in a xf96 microplate at a density of 5,000 cellswell 48h prior to experiment and maintained under standard tissue culture conditions with treatments of haase as indicated. The xf extracellular flux analyzer is the bestselling instrument. Seahorse xfe96 extracellular flux analyzer dartlab. The four corners were left only with medium for background correction. Seahorse xfe 24 extracellular flux analyzer based analysis of cellular respiration in caenorhabditis elegans. Seahorse xfe96 analyzer measures ocr and ecar of live cells in a 96well plate. Advances in the quantification of mitochondrial function. The seahorse bioscience xfp analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a. Sep 27, 2012 the cellular oxygen consumption rate ocr data were obtained using an xf96 extracellular flux analyzer from seahorse bioscience. Extracellular flux analysis to monitor glycolytic rates and mitochondrial oxygen consumption article in methods in enzymology 542.
Xf96 extracellular flux analyzer and provides basic instructions for bioenergetic data acquisition and. View and download seahorse xf96 installation and operation manual online extracellular flux analyzer and prep station xf96 oxygen equipment pdf manual download. Oxygen consumption rates ocr were measured using an xf96 extracellular flux analyzer. While spectrafoo has a few specialty tools such as the code metering, i feel pure analyzer excels in ease of use, flexibility, and responsiveness. Collection of cell analysis resources agilent community. The first thing that is different about analyzer, is unlike their other products that function as plugins within the host application like pro tools, analyzer doesnt. This product eliminates the need for radioactivity in fatty acid oxidation. Advances in the quantification of mitochondrial function in. Designed and delivered tumor cell metabolism assays with a new instrument xf96 extracellular flux analyzer unused priorly. Liver p53 is stabilized upon starvation and required for. This manual is a guide for the usage and functions of the flux.
Each person should firmly grasp the base of the unit at opposite ends to one another. Glycolysis is determined through measurements of the extracellular acidification rate ecar of the surrounding media, which is predominately from the excretion of lactic acid per unit time. Furthermore, changes in mitochondrial function may regulate lymphocyte function in inflammatory diseases like type 2 diabetes. To all potential users of the seahorse bioscience machines. Characterisationprocess equipphysical manufacturer seahorse biosceince inc.
It covers the interface views, connectivity and suggestions. Metabolic studies were performed using the xf96 extracellular flux analyzer seahorse bioscience, north billerica, killer et al. Bk precision 4052 function generator the 4052 is a. Consumables include flux paks, microplates, and stress kits. The advent of the seahorse xf24 and xf96 has not only allowed for rapid and highthroughput determination of. Setup and configure a020d teststandworkstationfor delta analyzer. The measurement of cellular bioenergetics on live cells enables timeresolved analysis and testing of multiple conditions per assay well. The user can make these waveforms singleshot, using a trigger source, or repetitive, based on use. See the complete profile on linkedin and discover margarets. Human induced pluripotent stem cells harbor homoplasmic.
Extracellular flux analysis to monitor glycolytic rates and. Xfp analyzer overview the seahorse bioscience xfp analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a miniplate. Seahorse xfe96 extracellular flux analyzer the seahorse enables quantitation of metabolomics of live cells in real time with stimulation with up to 4 drugs. They have also made the standalone application 64 bit compliant too. Results showed slowed growth and inhibition of glycolytic metabolism in. Open the seahorse xfe96 extracellular flux assay kit and remove the contents. This instrument measures oxygen consumption rate ocr and extracellular acidification rate ecar of living cells simultaneously in a 24 well format. Seahorse bioscience xf96 extracellular flux analyzer w xf. Our methodological approach relies on an extracellular flux analyzer, an instrument that enables the realtime measurement of the two central pathways used by living cells to generate adenosine triphosphate. The culture medium was replaced with 180 ml of bicarbonatefree dmem and cells were incubated at 37c for 30 min before measurement.
Seahorse xf96 manuals manuals and user guides for seahorse xf96. Seahorse xf96 installation and operation manual free reading at passwordsafe. Seahorse xfe24 extracellular flux analyzerbased analysis. Seahorse xf24 extracellular flux analyzer department of. Bioenergetic analysis of intact mammalian cells using the seahorse xf24 extracellular flux analyzer and a luciferase atp assay. Flux release another plugin pure analyser now 64bit. Required equipment, consumables, and software the following equipment, consumables, and software are required in addition to the materials specified in the icell cardiomyocytes users guide. The measurement of cellular bioenergetics on live cells enables timeresolved.
A function generator is a type of signal generator people use to generate many kinds of electrical waveforms over a range of frequencies. Within this ring, reentrant waves of action potential. The xf palmitatebsa fao reagent is a solution absorbed by cells. Extracellular flux analysis to monitor glycolytic rates. Do not apply values greater than the maximum value stated. Immobilization of nonadherent cells with celltak for assay on the agilent seahorse xfexf96 or xfp analyzer application notes. The seahorse xf24 is a revolutionary instrument for studies of cellular energy metabolism.
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